INSTRUCTIONS FOR USE

RETICULOCYTE STAINING AND COUNTING KIT

Staining of human clinical specimens, for subsequent visualisation by microscopy
For professional use only
Single use
Medical device for In Vitro Diagnostic

Intended Use:  Reticulocyte vital staining kit is intended for staining human clinical samples to determine the concentration of reticulocytes in circulating blood. It is used in medicine for cellular diagnostics and in the haematological examination of human samples.

General precaution:
1. Not suitable for any other application than its intended use.
2. Do not use the product if package is damaged.
3. Do not use the product if it has changed its original colour.
4. Do not use if the expiry date has been overcome.
5. Dispose as hazardous waste.
6. For its storage it is recommended to keep the product closed, at room temperature, in a well-ventilated area, and away from any hot or ignition point.

Reagents:
Code: 801000 – Box of 50 tubes.

Instructions for use:
1. Add 3 drops of blood to a tube containing 100 μl of stable stain.
2. Mix well and incubate for 10-15 minutes at room temperature.
3. Mix again the suspension and make two smears on the slide, let them dry at room temperature.
4. View through and oil immersion slide without further stain.

Results: Reticulocytes: precipitated RNA is reticular or granular and turns into intense blue.
Erythrocytes: turn into pale blue or green-blue.

Note: Care must be taken since stain particles that adhere on the surface of the small corpuscles can be misinterpreted. The coloration of preparations remains stable if they are not exposed to light.

Calculation: The percentage of erythrocytes containing reticules or granules is calculated. At least 1.000 erythrocytes in different parts of the extension must be observed. After calculating the percentage of reticulocytes, the total count of them can be determined multiplying the percentage for the erythrocytes count.
The standardised values are to be taken from current technical literature.

References:
1. John d. Bancroft, Marilyn Gamble. Theory and Practice of Histological Techniques, Churchill Livingstone Elsevier, Sixth Edition, 2008.
2. J. A. Kiernan et al. Histological & Histochemical Methods, Pergamon Press, Second Edition, 1990.
3. George Clark et al. Staining Procedures, Williams & Wilkins, Fourth Edition, 1981.